CD8 T Cells from a Novel T Cell Receptor Transgenic Mouse Induce Liver-Stage Immunity That Can Be Boosted by Blood-Stage Infection in Rodent Malaria

LS Lau; D Fernandez-Ruiz; V Mollard; A Sturm; MA Neller; A Cozijnsen; JL Gregory; GM Davey; CM Jones; YH Lin; A Haque; CR Engwerda; CQ Nie; DS Hansen; KM Murphy; AT Papenfuss; JJ Miles; SR Burrows; T de Koning-Ward; GI McFadden; FR Carbone; BS Crabb; WR Heath

Journal article

CD8 T Cells from a Novel T Cell Receptor Transgenic Mouse Induce Liver-Stage Immunity That Can Be Boosted by Blood-Stage Infection in Rodent Malaria

LS Lau, D Fernandez-Ruiz, V Mollard, A Sturm, MA Neller, A Cozijnsen, JL Gregory, GM Davey, CM Jones, YH Lin, A Haque, CR Engwerda, CQ Nie, DS Hansen, KM Murphy, AT Papenfuss, JJ Miles, SR Burrows, T de Koning-Ward, GI McFadden Show all

Plos Pathogens | PUBLIC LIBRARY SCIENCE | Published : 2014

DOI: 10.1371/journal.ppat.1004135

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Abstract

To follow the fate of CD8+ T cells responsive to Plasmodium berghei ANKA (PbA) infection, we generated an MHC I-restricted TCR transgenic mouse line against this pathogen. T cells from this line, termed PbT-I T cells, were able to respond to blood-stage infection by PbA and two other rodent malaria species, P. yoelii XNL and P. chabaudi AS. These PbT-I T cells were also able to respond to sporozoites and to protect mice from liver-stage infection. Examination of the requirements for priming after intravenous administration of irradiated sporozoites, an effective vaccination approach, showed that the spleen rather than the liver was the main site of priming and that responses depended on CD8α..

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University of Melbourne Researchers

Anton Cozijnsen Author

Gayle Davey Author

Ashraful Haque Author

Tony Papenfuss Author

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Awarded by National Health and Medical Research Council

Funding Acknowledgements

This work was supported by grants from the Australian Research Council (FF776174, CE140100011, DP130102481) and the National Health and Medical Research Council of Australia (APP1016629, APP1059861). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.